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BIOL 3900: Advanced Research in Life Sciences, Fall 2021

Glossary

Fatty acid amide hydrolase (FAAH): enzyme that cleaves the amide bond of multiple acyl amides, this results in the production of ethanolamine and free fatty acids.  

N-acylethanolamines (NAEs): lipophilic acyl amides with multiple biological roles in animals and plants. Binary designation NAE(X):(Y) is the most common nomenclature, where X and Y represent the length of the acyl chain linked to ethanolamine and the number of double bonds in the acyl moiety, respectively. For example; NAE18:1, NAE18:2, and NAE20:4.  

Anandamide (NAE20:4):  NAE in mammalian systems known to be involved in multiple neurological processes. In non-vascular plants (e.g. mosses) this NAE has also been detected, but role is yet to be determined.  

N-3-(oxododecanoyl)-l-homoserine lactone (OdDHL): is a quorum sensing molecule that enables bacteria to bacteria communication.  

Fluorescamine: reagent that is used for detection of primary amines, it is not fluorescent by itself but upon reaction with a primary amine (e.g. ethanolamine), it results in the formation of a fluorescent complex.  

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): polyacrylamide gel system to separate proteins by their mass  

Fast protein liquid chromatography (FPLC): chromatography method to purify proteins.  

Recombinant protein: protein that results of the translation from a foreign gene into a host expression system (e.g. bacteria, or yeast).   

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